The continuous and single pulses exposure of Cu, Zn, Pb and Cd on larvae Brachymystax lenok were tested in this study. The first-order kinetics was employed to obtain better comprehension on the time-toxicity relationship of tested heavy metals in continuous exposure test. For difference in time-varying exposure or recovery, 50% lethal time-averaged concentration (TAC) was used to assess the pulsed toxicity, which took both time and concentration into consideration in exposure and post-exposure period. TAC assessment to the single pulses and the regression analysis of first-order kinetic to continuous exposures demonstrated that Cu and Zn showed greater toxicity than Pb and Cd, indicating a slower uptake and/or depuration effect for zinc or copper. Furthermore, the results showed that recovery effect of larvae to all four metals was found in 24 h exposure followed by 72 h in fresh water, while for 72 h exposure and 24 h non-exposure the delayed lethal phenomenon occurred.
Keywords: Brachymystax lenok; Delayed effect; First-order kinetic equation; Heavy metal; Single pulses exposure; TAC.
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