Changes in Some Divalent Cations Concentrations In An Experimental Rat Model of Gentamicin-Induced Acute Renal Failure

Rev Med Chir Soc Med Nat Iasi. 2016 Oct-Dec;120(4):947-52.

Abstract

Aim: The changes in divalent cations concentration were assessed in and experimentally gentamicin-induced renal failure in white Wistar rats.

Material and methods: The white male Wistar rats were distributed into 4 groups of 7 animals each and were treated intraperitoneally as follows: Group I (Control): distilled water in a volume of 0.5ml/100g rat/day for10 days; Group II (Ge): gentamicin 80 mg/kbw/day for 7 days; Group III (Ge+Zn): gentamicin 80 mg/kbw/day for 7 days and ZnCl2 5 mg/kbw/day for 10 days prior to administration of Ge and then another 7 days simultaneously with Ge administration; Group IV (Zn): ZnCl2 5 mg/kbw/day for 17 days. Before starting the experiment (I0) and at 3, 7 and 10 days after the first Ge administration, magnesium, copper and zinc plasma concentrations and urinary magnesium levels were determined.

Results: Zn administration significantly decreased (p<0.001) plasma Mg concentrations in Ge+Zn group compared to Ge group after 7 days in the experiment, and induced a lower urinary elimination of Mg in Ge+Zn group (p<0.05) than in Ge group (p<0.01). Also, Zn induced a slight augmentation of Cu concentration in Ge+Zn group (p<0.05) compared to Ge group after 7 and 10 days.

Conclusions: The variation in divalent cation concentrations in the context of renal diseases may be helpful for an early diagnosis and effective alternative therapeutic measures.

Publication types

  • Comparative Study

MeSH terms

  • Acute Kidney Injury / blood*
  • Acute Kidney Injury / chemically induced
  • Acute Kidney Injury / urine
  • Animals
  • Anti-Bacterial Agents*
  • Biomarkers / blood
  • Cations, Divalent / blood*
  • Cations, Divalent / urine
  • Copper / blood
  • Disease Models, Animal
  • Gentamicins*
  • Magnesium / blood
  • Rats
  • Rats, Wistar
  • Trace Elements / blood*
  • Trace Elements / urine
  • Zinc / blood

Substances

  • Anti-Bacterial Agents
  • Biomarkers
  • Cations, Divalent
  • Gentamicins
  • Trace Elements
  • Copper
  • Magnesium
  • Zinc