The proliferative immune response to autologous Epstein-Barr virus transformed lymphoblastoid cells. II. Studies with HLA class II loss variants demonstrate a role for gene products other than DR and DQ

Hum Immunol. 1986 Jul;16(3):276-88. doi: 10.1016/0198-8859(86)90055-8.

Abstract

The Epstein-Barr virus (EBV) transformed lymphoblastoid cell line (LCL-721) and some of its HLA loss mutant derivatives were used to study the immune specificity of the autologous proliferative T cell response to antigens expressed as a result of EBV infection. We have measured secondary and tertiary proliferative responses to well-characterized variants that lack expression of some or all known class II gene products (DR, DQ, and DP). These experiments prove that the region mapping between DR/DQ and glyoxalase I (GLO) of one haplotype controls at least one specific restriction element which is recognized in the autologous response to LCL-721. Furthermore, specific proliferative responses to variants lacking expression of all known class II gene products indicate the recognition of determinants other than DR, DQ, and DP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Transformation, Viral
  • Genes, MHC Class II*
  • Genetic Variation
  • HLA-DQ Antigens
  • HLA-DR Antigens
  • Herpesvirus 4, Human / immunology*
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Lymphocyte Activation*
  • Lymphocytes / immunology*
  • Major Histocompatibility Complex

Substances

  • HLA-DQ Antigens
  • HLA-DR Antigens
  • Histocompatibility Antigens Class II