Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes

Chem Commun (Camb). 2018 Aug 23;54(69):9627-9630. doi: 10.1039/c8cc03713b.

Abstract

We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N6-methyladenosine (m6A)-containing RNA and unmethylated RNA. By applying this principle, we quantified methylation efficiency at m6A sites in E. coli 23S rRNA with high accuracy.

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / chemistry
  • DNA Probes / genetics*
  • Escherichia coli / metabolism
  • Methylation
  • Nucleic Acid Hybridization
  • RNA, Ribosomal, 23S / chemistry
  • RNA, Ribosomal, 23S / genetics
  • RNA, Ribosomal, 23S / metabolism*
  • Transition Temperature

Substances

  • DNA Probes
  • RNA, Ribosomal, 23S
  • N-methyladenosine
  • Adenosine