Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS)

Joanna Serwatowska; Ole Søgaard Lund; Ida Elisabeth Johansen

doi:10.1007/978-1-4939-8633-0_8

Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS)

Methods Mol Biol. 2018:1822:115-122. doi: 10.1007/978-1-4939-8633-0_8.

Authors

Joanna Serwatowska¹, Ole Søgaard Lund², Ida Elisabeth Johansen³

Affiliations

¹ Unidad de Genómica Avanzada (LANGEBIO), CINVESTAV-IPN, Guanajuato, Mexico.
² Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
³ Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark. ejoh@plen.ku.dk.

PMID: 30043300
DOI: 10.1007/978-1-4939-8633-0_8

Abstract

Successful application of virus-induced gene silencing for functional genomics requires a virus vector that can initiate a systemic infection of the host plant. Agroinoculation of the pea early browning virus vectors pCAPE1 and pCAPE2 can establish infection in several genotypes of Medicago truncatula and can reduce target gene RNA levels to an extent that allows investigation of gene function.

Keywords: Functional genomics; PEBV; Pea early browning virus; Reverse genetics; Virus vector.

MeSH terms

Gene Expression Regulation, Plant*
Gene Silencing*
Genome, Plant
Genomics / methods
Medicago truncatula / genetics*
Medicago truncatula / microbiology
Phenotype
Plants, Genetically Modified
Plasmids / genetics
RNA Interference*