Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula

Methods Mol Biol. 2018:1822:71-82. doi: 10.1007/978-1-4939-8633-0_5.

Abstract

TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18:455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest.

Keywords: EMS chemical mutagenesis; Medicago truncatula; Reverse genetics; TILLING.

MeSH terms

  • Ethyl Methanesulfonate / pharmacology
  • Genome, Plant*
  • Genomics* / methods
  • Medicago truncatula / drug effects
  • Medicago truncatula / genetics*
  • Mutagenesis / drug effects
  • Nucleic Acid Amplification Techniques
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Reproducibility of Results
  • Seeds / drug effects
  • Seeds / genetics

Substances

  • Ethyl Methanesulfonate