Self-Reporting Chemically Induced Protein Proximity System Based on a Malachite Green Derivative and the L5** Fluorogen Activating Protein

Bioconjug Chem. 2018 Sep 19;29(9):3010-3015. doi: 10.1021/acs.bioconjchem.8b00415. Epub 2018 Aug 20.

Abstract

A unique chemically induced proximity method is engineered based on mutant antibody VL domain using a fluorogenic malachite green derivative as the inducer, which gives fluorescent signals upon VL domain dimerization while simultaneously inducing downstream biological effects.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Dimerization
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • HEK293 Cells
  • Humans
  • Protein Domains
  • Proteins / chemistry*
  • Rosaniline Dyes / chemistry*

Substances

  • Fluorescent Dyes
  • Proteins
  • Rosaniline Dyes
  • malachite green