Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

Future Microbiol. 2018 Sep:13:1225-1246. doi: 10.2217/fmb-2018-0082. Epub 2018 Jun 25.

Abstract

Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan.

Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping.

Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48).

Conclusion: Our prevalence data from Pakistan show that - as in other parts of the world - carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

Keywords: CPO; CarbDetect; ESBL; Pakistan; carbapenemases; hospital; microarray.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics*
  • Genotype
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / genetics*
  • Gram-Negative Bacteria / isolation & purification*
  • Humans
  • Kidney Transplantation
  • Microbial Sensitivity Tests
  • Oligonucleotide Array Sequence Analysis*
  • Pakistan
  • Phenotype
  • Tertiary Care Centers
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • beta-Lactamases
  • carbapenemase