Abstract
A protein fraction containing neurofilaments was prepared from rat brain cytosol by differential centrifugation and gel filtration chromatography. These preparations were enriched for a calcium/calmodulin-dependent kinase activity that phosphorylated endogenous neurofilament proteins. The enzyme incorporated approximately 1 mol PO4/mol of each neurofilament triplet polypeptide. These data suggest that a calmodulin-dependent kinase may mediate some of the effects of calcium on cytoskeletal function by phosphorylation of neurofilament proteins.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Brain / enzymology*
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Calmodulin / isolation & purification
-
Cattle
-
Cytosol / enzymology
-
Electrophoresis, Polyacrylamide Gel
-
Intermediate Filament Proteins / isolation & purification
-
Intermediate Filament Proteins / metabolism*
-
Kinetics
-
Molecular Weight
-
Neurofilament Proteins
-
Phosphorylation
-
Protein Kinases / metabolism*
-
Rats
-
Spinal Cord / metabolism
Substances
-
Calmodulin
-
Intermediate Filament Proteins
-
Neurofilament Proteins
-
Protein Kinases
-
Calcium-Calmodulin-Dependent Protein Kinases