Design and evaluation of nested PCR primers for specific detection of genogroup I noroviruses in oysters

Ping Guo; Yongxin Yu; Yingjie Pan; Shuling Yan; Yongjie Wang

doi:10.1016/j.mcp.2018.06.002

Design and evaluation of nested PCR primers for specific detection of genogroup I noroviruses in oysters

Mol Cell Probes. 2018 Aug:40:40-43. doi: 10.1016/j.mcp.2018.06.002. Epub 2018 Jun 11.

Authors

Ping Guo¹, Yongxin Yu², Yingjie Pan², Shuling Yan³, Yongjie Wang⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China.
³ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China. Electronic address: yjwang@shou.edu.cn.

PMID: 29902509
DOI: 10.1016/j.mcp.2018.06.002

Abstract

A pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters.

Keywords: Coverage; GI norovirus; Oyster; Primer; Sensitivity; Specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Computer Simulation
DNA Primers / metabolism*
Humans
Norovirus / genetics*
Norovirus / isolation & purification*
Ostreidae / virology*
Polymerase Chain Reaction / methods*

Substances

DNA Primers