Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans

Methods Mol Biol. 2018:1797:217-232. doi: 10.1007/978-1-4939-7883-0_11.

Abstract

Environmental toxicants, chemical substances produced or introduced into the environment directly by humans or their activities, can act as teratogens during development that negatively impact health. Long-term ramifications of environmental exposures to sublethal doses of teratogens are often unrecognized and unknown. The round worm, Caenorhabditis elegans, is an emerging model organism to investigate the long-term impacts of environmental teratogens upon health. This chapter describes a toxicant exposure paradigm integrated with phenotyping assays to screen adult worms, and their progeny, for effects on reproduction, growth and development, behavior, and energy balance.

Keywords: C. elegans; Growth; Long-term health effects; Obesity; Reproduction; Subtoxic exposure.

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / growth & development*
  • Energy Metabolism
  • Environmental Exposure / adverse effects*
  • Phenotype
  • Reproduction
  • Teratogens / toxicity*
  • Toxicity Tests / methods*

Substances

  • Teratogens