Abstract
The single site of integration of hepatitis B virus in the human hepatocellular carcinoma cell line Hep 3B 2-1/7 was found to segregate with human chromosome 12 in somatic cell hybrids. Analysis of metaphase spreads of Hep 3B 2-1/7 following in situ hybridization with pHBV revealed integration at 12q13----q14, a location that coincides with a fragile site, fra (12q13). The possible significance of this location to the development of hepatocellular carcinomas is discussed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / microbiology
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Cell Line
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Chromosomes, Human, 6-12 and X*
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Hepatitis B Surface Antigens / genetics
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Hepatitis B virus / genetics*
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Humans
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Hybrid Cells / cytology
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Karyotyping
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Liver Neoplasms / genetics*
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Liver Neoplasms / microbiology
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Liver Neoplasms, Experimental / genetics
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Rats
Substances
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Hepatitis B Surface Antigens