Two-photon speckle illumination for super-resolution microscopy

J Opt Soc Am A Opt Image Sci Vis. 2018 Jun 1;35(6):1028-1033. doi: 10.1364/JOSAA.35.001028.

Abstract

We present a numerical study of a microscopy setup in which the sample is illuminated with uncontrolled speckle patterns and the two-photon excitation fluorescence is collected on a camera. We show that, using a simple deconvolution algorithm for processing the speckle low-resolution images, this wide-field imaging technique exhibits resolution significantly better than that of two-photon excitation scanning microscopy or one-photon excitation bright-field microscopy.