Synthesis of Metal-Organic Frameworks on Tobacco Mosaic Virus Templates

Methods Mol Biol. 2018:1798:95-108. doi: 10.1007/978-1-4939-7893-9_8.

Abstract

Tobacco mosaic virus (TMV) has long been exploited as a robust biological scaffold for organic/inorganic modification owing to its anisotropic structure and chemically addressable amino acid residues on both the exterior and interior. We present the fabrication of a crystalline microporous metal-organic framework (MOF) shell on the exterior of TMV, which retains its rod-like morphology, and produces uniformly formed core-shell structures with high accessible surface area and pore volume. We also describe an exfoliation method that can recover the intact viral particle from the core-shell composite.

Keywords: Biomimetic mineralization; Chemical virology; MOFs; Metal–organic frameworks; SURMOFs; Self-assembly; Templated crystal growth; Tobacco mosaic virus; VLPs; Virus nanotechnology; Virus-like particles.

MeSH terms

  • Capsid Proteins / chemistry
  • Capsid Proteins / genetics
  • Capsid Proteins / isolation & purification
  • Capsid Proteins / metabolism
  • Metal Nanoparticles* / chemistry
  • Metal-Organic Frameworks / chemical synthesis*
  • Metal-Organic Frameworks / chemistry
  • Models, Molecular
  • Nanotechnology
  • Protein Conformation
  • Recombinant Fusion Proteins
  • Tobacco Mosaic Virus* / chemistry
  • Tobacco Mosaic Virus* / ultrastructure
  • Virion / chemistry
  • Virion / ultrastructure
  • Virus Assembly
  • X-Ray Diffraction

Substances

  • Capsid Proteins
  • Metal-Organic Frameworks
  • Recombinant Fusion Proteins