Salt-stable association of simian virus 40 capsid with simian virus 40 DNA

FEBS Lett. 1985 Feb 11;181(1):64-8. doi: 10.1016/0014-5793(85)81114-5.

Abstract

In 8 M CsCl, a fraction of the wild-type previrions and tsB228 nucleoprotein complexes lose their core histones but retain their capsid. These histone-depleted complexes appear in the electron microscope as a protein shell attached to supercoiled DNA. Consistent with this result, we find that in 1 M NaCl, the wild-type previrions dissociate into two populations of nucleoprotein complexes. One population sediments between 50 and 140 S and morphologically resembles the shell-DNA complexes isolated in CsCl gradients. The other population is comprised primarily of nucleoproteins which sediment at 40 S.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Capsid / metabolism*
  • Centrifugation, Density Gradient
  • Chromosomal Proteins, Non-Histone / analysis
  • DNA, Superhelical / metabolism
  • DNA, Viral / metabolism*
  • Microscopy, Electron
  • Nucleoproteins / analysis
  • Simian virus 40

Substances

  • Chromosomal Proteins, Non-Histone
  • DNA, Superhelical
  • DNA, Viral
  • Nucleoproteins