Metagenomic Description of Preenrichment and Postenrichment of Recalled Chapati Atta Flour Using a Shotgun Sequencing Approach

Tina Lusk Pfefer; Padmini Ramachandran; Elizabeth Reed; Julie A Kase; Andrea Ottesen

doi:10.1128/genomeA.00305-18

Metagenomic Description of Preenrichment and Postenrichment of Recalled Chapati Atta Flour Using a Shotgun Sequencing Approach

Genome Announc. 2018 May 24;6(21):e00305-18. doi: 10.1128/genomeA.00305-18.

Authors

Tina Lusk Pfefer¹, Padmini Ramachandran², Elizabeth Reed², Julie A Kase², Andrea Ottesen²

Affiliations

¹ Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, Maryland, USA tina.pfefer@fda.hhs.gov.
² Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, Maryland, USA.

Abstract

The bacterial microbiome of flour recalled for possible Escherichia coli O121 contamination was characterized before (hour 0) and after (hour 24) enrichment using shotgun sequencing. At hour 0, Staphylococcus (46.8 to 66.5%) and Pantoea (12.6 to 21.0%) bacteria were dominant. At hour 24, Enterobacter (28.7 to 70.9%) and Klebsiella (25.6 to 68.6%) bacteria dominated, and Escherichia coli ranged from 0.3 to 17.9%.