Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma

Zheng Wang; Xiaonan Wu; Yuankai Shi; Xiaohong Han; Gang Cheng; Di Cui; Lin Li; Yuhui Zhang; Xinlin Mu; Li Zhang; Li Yang; Jing Di; Qi Yu; Dongge Liu

doi:10.2217/pme.15.3

Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma

Per Med. 2015 Aug;12(4):349-357. doi: 10.2217/pme.15.3. Epub 2015 Feb 4.

Authors

Zheng Wang¹, Xiaonan Wu², Yuankai Shi³, Xiaohong Han³, Gang Cheng², Di Cui¹, Lin Li², Yuhui Zhang⁴, Xinlin Mu⁵, Li Zhang⁶, Li Yang¹, Jing Di¹, Qi Yu¹, Dongge Liu¹

Affiliations

¹ Department of Pathology, Beijing Hospital of the Ministry of Health, Beijing, China.
² Department of Medical Oncology, Beijing Hospital of the Ministry of Health, Beijing, China.
³ Department of Medical Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Beijing, China.
⁴ Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
⁵ Department of Respiratory & Critical Care Medicine, Peking University People's Hospital, Beijing, China.
⁶ Department of Respiratory Medicine, Peking Union Medical College Hospital, Beijing, China.

PMID: 29771657
DOI: 10.2217/pme.15.3

Abstract

Aim: To evaluate the Ventana IHC ALK (D5F3) assay for detecting anaplastic lymphoma kinase (ALK) protein expression in pleural effusion samples.

Methods: Historical, selected (wild-type EGFR, K-RAS) pleural effusion cytologic blocks of lung adenocarcinoma samples (Study 1) and unselected lung adenocarcinoma pleural effusion cytologic blocks (Study 2) were tested by Ventana IHC ALK (D5F3) assay. Quantitative real-time-PCR was used to verify immunohistochemistry results.

Results: A total of 17 out of 100 (Study 1) and ten out of 104 (Study 2) pleural effusion samples were ALK expression positive by the Ventana IHC ALK (D5F3) assay. The ALK fusion results with immunohistochemistry and quantitative real-time-PCR had a concordance rate of 87.5% (κ = 0.886; p < 0.001).

Conclusion: The Ventana IHC ALK (D5F3) assay is a reliable tool for detecting ALK protein expression in pleural effusion samples.

Keywords: ALK; NSCLC; diagnosis; fluorescence in situ hybridization; immunohistochemistry; lung cancer; qRT-PCR.