[The role of autophagy in the invasion and metastasis of the squamous cell carcinoma of head and neck]

C H Chen; G C Zhu; L M Pi; M Wei; L She; H L Tan; G C Liu; Y Liu; X Zhang

doi:10.13201/j.issn.1001-1781.2017.10.013

[The role of autophagy in the invasion and metastasis of the squamous cell carcinoma of head and neck]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 May 20;31(10):789-793. doi: 10.13201/j.issn.1001-1781.2017.10.013.

[Article in Chinese]

Authors

C H Chen¹, G C Zhu², L M Pi¹, M Wei¹, L She¹, H L Tan³, G C Liu¹, Y Liu¹, X Zhang¹

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
² Department of Otolaryngology Head and Neck Surgery, the Second Xiangya Hospital, Central South University.
³ Department of Head and Neck Surgery, Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University.

PMID: 29771045
DOI: 10.13201/j.issn.1001-1781.2017.10.013

Abstract

Objective:The aim of this study is to explore the effects of autophagy on the metastasis of the Squamous Cell Carcinoma of Head and Neck (SCCHN) via epithelial to mesenchymal transition (EMT) induced by TGF-β1. Method:Establish the EMT model induced by TGF-β1 in the SCCHN in time/concentration, and the expression of autophagy related protein microtubule associated protein 1 light chain3 (LC3) detected by western blot; Autophagy inhibitor chloroquine (CQ), depressing autophagy, the expression of E-cadherin, cytokeratin, Vimentin and LC3 were examined by Western blot. Wound healing and Transwell invasion assay indicate the effects to metastasis for SCCHN. Result:Autophagy was activated within TGF-β1 induced EMT model in the SCCHN in time/concentration dependently. After autophagy was suppressed, the expression of E-cadherin and cytokeratin increased while vimentin and the capacity of metastasis was reduced compared with control group. Conclusion:TGF-β1 induce EMT and Autophagy in the SCCHN. Autophagy could enhances metastasis in the SCCHN via EMT induced by TGF-β1.

Keywords: Epithelial to mesenchymal transition; TGF-β1; autophagy; squamous cell carcinoma of head and neck.

MeSH terms

Autophagy*
Biomarkers, Tumor*
Cadherins
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Epithelial-Mesenchymal Transition
Head and Neck Neoplasms / pathology*
Humans
Neoplasm Invasiveness*
Transforming Growth Factor beta1

Substances

Biomarkers, Tumor
Cadherins
Transforming Growth Factor beta1