Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling

Samuel A Myers; Jason Wright; Ryan Peckner; Brian T Kalish; Feng Zhang; Steven A Carr

doi:10.1038/s41592-018-0007-1

Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling

Nat Methods. 2018 Jun;15(6):437-439. doi: 10.1038/s41592-018-0007-1. Epub 2018 May 7.

Authors

Samuel A Myers¹, Jason Wright², Ryan Peckner², Brian T Kalish^{3

4}, Feng Zhang², Steven A Carr⁵

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, MA, USA. sammyers@broadinstitute.org.
² Broad Institute of MIT and Harvard, Cambridge, MA, USA.
³ Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
⁴ Division of Newborn Medicine, Department of Medicine, Boston Children's Hospital, Boston, MA, USA.
⁵ Broad Institute of MIT and Harvard, Cambridge, MA, USA. scarr@broad.mit.edu.

Abstract

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Associated Protein 9 / metabolism*
Chromatin Immunoprecipitation
DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism*
Endonucleases
Genome
Genomics
HEK293 Cells
Humans
Multifunctional Enzymes
Polymerase Chain Reaction
Protein Engineering

Substances

Multifunctional Enzymes
CRISPR-Associated Protein 9
Endonucleases
APEX2 protein, human
DNA-(Apurinic or Apyrimidinic Site) Lyase

Abstract

Publication types

MeSH terms

Substances

Grants and funding