First isolation of West Nile virus in Zambia from mosquitoes

Y Orba; B M Hang'ombe; A S Mweene; Y Wada; P D Anindita; W Phongphaew; Y Qiu; M Kajihara; A Mori-Kajihara; Y Eto; M Sasaki; W W Hall; Y Eshita; H Sawa

doi:10.1111/tbed.12888

First isolation of West Nile virus in Zambia from mosquitoes

Transbound Emerg Dis. 2018 Aug;65(4):933-938. doi: 10.1111/tbed.12888. Epub 2018 May 2.

Authors

Y Orba¹, B M Hang'ombe², A S Mweene³, Y Wada¹, P D Anindita¹, W Phongphaew¹, Y Qiu⁴, M Kajihara⁵, A Mori-Kajihara⁵, Y Eto⁵, M Sasaki¹, W W Hall^{6

7

8}, Y Eshita⁴, H Sawa^{1

7

8}

Affiliations

¹ Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
² Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
³ Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
⁴ Hokudai Center for Zoonosis Control in Zambia, Lusaka, Zambia.
⁵ Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
⁶ Centre for Research in Infectious Diseases, University College Dublin, Dublin, Ireland.
⁷ Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan.
⁸ Global Virus Network, Baltimore, Maryland, USA.

PMID: 29722174
DOI: 10.1111/tbed.12888

Abstract

Mosquito surveillance studies to identify mosquito-borne flaviviruses have identified West Nile Virus (WNV) for the first time in Zambia. The Zambian WNV isolate from Culex quinquefasciatus mosquitoes collected in the Western Province was closely related genetically to WNV lineage 2 South African strains which have been previously shown to be highly neuroinvasive. These data provide the first evidence of the circulation of WNV in Zambia and suggest there should be an increased awareness of possible associated human and animal diseases in that country.

Keywords: West Nile virus; Zambia; arbovirus; mosquito.

MeSH terms

Animals
Chlorocebus aethiops
Cricetinae
Culex / virology*
Flavivirus / isolation & purification
Humans
Insect Vectors / virology
Kidney / cytology
Real-Time Polymerase Chain Reaction
Vero Cells
West Nile Fever / epidemiology
West Nile Fever / virology*
West Nile virus / genetics
West Nile virus / isolation & purification*
Zambia / epidemiology

Associated data

GENBANK/LC318700
GENBANK/AF260969
GENBANK/AF404757
GENBANK/AY842931
GENBANK/EU081844
GENBANK/KX394398
GENBANK/DQ256376
GENBANK/JN393308
GENBANK/EF429197
GENBANK/EF429198
GENBANK/EF429200
GENBANK/EF429199
GENBANK/KM052152
GENBANK/KX375812
GENBANK/DQ116961
GENBANK/DQ318019
GENBANK/ArD76104
GENBANK/AY532665
GENBANK/DQ176636
GENBANK/AY765264
GENBANK/AY277251