In vitro selection of electrochemical peptide probes using bioorthogonal tRNA for influenza virus detection

Chem Commun (Camb). 2018 May 17;54(41):5201-5204. doi: 10.1039/c8cc01775a.

Abstract

An electrosensitive peptide probe has been developed from an in vitro selection technique using biorthogonal tRNA prepared with an electroreactive non-natural amino acid, 3,4-ethylenedioxythiophene-conjugated aminophenylalanine. The selected probe quantitatively detected the influenza virus based on a signal "turn-on" mechanism. The developed strategy could be used to develop electrochemical biosensors toward a variety of targets.

MeSH terms

  • Biosensing Techniques*
  • Electrochemical Techniques*
  • Molecular Probes / chemistry*
  • Orthomyxoviridae / isolation & purification*
  • Peptides / chemistry*
  • RNA, Transfer / chemistry*

Substances

  • Molecular Probes
  • Peptides
  • RNA, Transfer