Localization of the receptor binding site of IFN-alpha 2b

J Immunol. 1988 Sep 1;141(5):1550-5.

Abstract

The receptor binding site of IFN-alpha is not precisely known. To further characterize this site, mAb against IFN-alpha 2b were selected that block the binding of radiolabeled IFN-alpha 2b to its cell surface receptor. These antibodies also neutralized the anti-viral and anti-proliferative properties of IFN-alpha 2b. A subset of these antibodies (group 1) do not recognize IFN-alpha 2a, either in solid-phase immunoassays or functional assays, whereas a second subset (group 2), with no cross-reactivity with group 1, recognizes both IFN-alpha subtypes. Because IFN-alpha 2b and IFN-alpha 2a differ by only alpha Arg23-Lys23 substitution, group 1 antibodies must recognize an epitope within the receptor binding region of IFN-alpha 2b that includes Arg23. Group 2 antibodies recognize a separate and distinct epitope within the binding site that does not include Arg23.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / physiology
  • Binding Sites, Antibody
  • Binding, Competitive
  • Cell Line
  • Cross Reactions
  • Female
  • Growth Inhibitors / pharmacology
  • Immunoglobulin Fab Fragments / physiology
  • Interferon Type I / immunology
  • Interferon Type I / metabolism*
  • Interferon Type I / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Radioimmunoassay
  • Receptors, Immunologic / immunology
  • Receptors, Immunologic / metabolism*
  • Receptors, Interferon

Substances

  • Antibodies, Monoclonal
  • Growth Inhibitors
  • Immunoglobulin Fab Fragments
  • Interferon Type I
  • Receptors, Immunologic
  • Receptors, Interferon