Topical Application of Keratinocyte Growth Factor Conjugated Gold Nanoparticles Accelerate Wound Healing

Anqiang Pan; Meile Zhong; Hong Wu; Yi Peng; Hansong Xia; Qiyu Tang; Qiangru Huang; Lin Wei; Lehui Xiao; Cheng Peng

doi:10.1016/j.nano.2018.04.007

Topical Application of Keratinocyte Growth Factor Conjugated Gold Nanoparticles Accelerate Wound Healing

Nanomedicine. 2018 Jul;14(5):1619-1628. doi: 10.1016/j.nano.2018.04.007. Epub 2018 Apr 23.

Authors

Anqiang Pan¹, Meile Zhong², Hong Wu³, Yi Peng⁴, Hansong Xia⁴, Qiyu Tang⁵, Qiangru Huang⁵, Lin Wei⁶, Lehui Xiao⁷, Cheng Peng⁸

Affiliations

¹ The 3rd Xiangya Hospital, Central South University, Changsha, China; School of Materials Science and Engineering, Central South University, Changsha, China.
² State Key Laboratory of Medicinal Chemical Biology College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, China; Dynamic Optical Microscopic Imaging Laboratory, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China.
³ State Key Laboratory for Powder Metallurgy, Central South University, Changsha, China.
⁴ The 3rd Xiangya Hospital, Central South University, Changsha, China; Xiangya Medical School, Central South University, Changsha, China.
⁵ Xiangya Medical School, Central South University, Changsha, China.
⁶ Dynamic Optical Microscopic Imaging Laboratory, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China.
⁷ State Key Laboratory of Medicinal Chemical Biology College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, China; Dynamic Optical Microscopic Imaging Laboratory, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China.. Electronic address: lehuixiao@163.com.
⁸ The 3rd Xiangya Hospital, Central South University, Changsha, China. Electronic address: pcheng83@sina.com.

PMID: 29698728
DOI: 10.1016/j.nano.2018.04.007

Abstract

Keratinocyte growth factor (KGF) has been demonstrated to specifically stimulate the multiplication and migration of keratinocytes. However, due to rapid degradation, the results of topical application of growth factors on wounds are unsatisfactory. In this study, we cross-linked KGF to the surface of gold nanoparticles (GNPs) and explored their effects on wound healing. The as-synthesized nanocomposite (KGF-GNPs) displayed good colloidal stability, decent biocompatibility as well as negligible cellular cytotoxicity. The in vitro cellular experimental results demonstrated that KGF-GNPs could effectively promote the proliferation of keratinocytes in contrast to bare GNPs or KGF. Furthermore, in animal full-thickness wound model, KGF-GNPs are more conducive to wound healing than bare GNPs or KGF. KGF-GNPs enhanced wound healing by promoting wound re-epithelialization rather than granulation. The superior biocompatibility, colloidal depressiveness and biological activity of this nanocomposite indicate that it could be utilized as a promising wound healing drug for clinical application in the future.

Keywords: Gold nanoparticles; Keratinocyte growth factor; Re-epithelialization; Wound healing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Topical
Animals
Cells, Cultured
Female
Fibroblast Growth Factor 7 / administration & dosage*
Fibroblast Growth Factor 7 / chemistry
Gold / chemistry*
Keratinocytes / cytology*
Keratinocytes / drug effects
Metal Nanoparticles / administration & dosage*
Metal Nanoparticles / chemistry
Rats
Rats, Sprague-Dawley
Wound Healing*

Substances

Fibroblast Growth Factor 7
Gold