Establishment of STUB1/CHIP mutant induced pluripotent stem cells (iPSCs) from a patient with Gordon Holmes syndrome/SCAR16

Stem Cell Res. 2018 May:29:166-169. doi: 10.1016/j.scr.2018.04.001. Epub 2018 Apr 9.

Abstract

STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Here, we reprogrammed human skin fibroblasts from a 12-year-old male patient with recessive spinocerebellar ataxia type 16 (OMIM #615768), carrying compound heterozygous mutations (c.355C>T, c.880A>T) in STUB1. Genomic integrity of the iPSC line HIHCNi001-A without transgene integration and genomic aberration but with maintained disease-relevant mutations was proven by SNP array analysis and Sanger sequencing while pluripotency was verified by the expression of important pluripotency markers and the capacity to differentiate into cells of all three germ layers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellular Reprogramming Techniques*
  • Cerebellar Ataxia* / genetics
  • Cerebellar Ataxia* / metabolism
  • Cerebellar Ataxia* / pathology
  • Child
  • DNA Mutational Analysis
  • Fibroblasts / metabolism
  • Fibroblasts / pathology
  • Gonadotropin-Releasing Hormone / deficiency*
  • Gonadotropin-Releasing Hormone / genetics
  • Gonadotropin-Releasing Hormone / metabolism
  • Humans
  • Hypogonadism* / genetics
  • Hypogonadism* / metabolism
  • Hypogonadism* / pathology
  • Induced Pluripotent Stem Cells* / metabolism
  • Induced Pluripotent Stem Cells* / pathology
  • Male
  • Mutation*
  • Polymorphism, Single Nucleotide*
  • Skin / metabolism
  • Skin / pathology
  • Ubiquitin-Protein Ligases* / genetics
  • Ubiquitin-Protein Ligases* / metabolism

Substances

  • Gonadotropin-Releasing Hormone
  • STUB1 protein, human
  • Ubiquitin-Protein Ligases

Supplementary concepts

  • Cerebellar Ataxia and Hypogonadotropic Hypogonadism