Objective: To investigate a modified protocol of establishing mouse Ph+ ALL model so as to provide a more convenient and more powerful tool for Ph+ ALL studies.
Methods: Immature B cells from BALB/c mice were transfected with the Mig190 retrovirus and infused into irradiated syngeneic mice. The immunophenotype was identified by flow cytometry, the BCR-ABL was identified by RT-PCR and Western blot. Leukemia cells isolated from sick mice were re-infused into syngeneic mice without irradiation.
Results: The acute lymphoblastic leukemia was established in mice after Mig190 retroviral transfection, the lymphoblasts were observed by blood smears, the immunophenotype of these leukemic cells was CD19+, and the BCR-ABL was detected by RT-PCR and Western blot, the immunophenotype was conformed as Ph+ ALL, the isolated leukemia cells infused into mice rapidly developed B-ALL.
Conclusion: A new protocol is established to generate mice with Ph+ ALL. This mouse model can provide a more simple and effective tool in comparison with the conversional protocols.