Effects of acetone extract of Cola nitida on brain sodium-potassium adenosine triphosphatase activity and spatial memory in healthy and streptozotocin-induced diabetic female Wistar rats

J Basic Clin Physiol Pharmacol. 2018 Jul 26;29(4):411-416. doi: 10.1515/jbcpp-2016-0019.

Abstract

Background This study was carried out to investigate the effects of acetone extract of Cola nitida on brain Na+/K+-ATPase activity and spatial memory of healthy and streptozotocin (STZ)-induced diabetic female Wistar rats. Methods Forty-two female Wistar rats were used for this study and were randomly distributed into six groups (n=7). Rats in group 1 were used as control and were administered normal saline; group 2 rats were healthy rats administered 50 mg/kg of kola nut extract per day; group 3 rats were healthy rats administered 100 mg/kg of kola nut extract per day; group 4 rats were a diabetic group also administered normal saline; group 5 rats were diabetic rats administered 50 mg/kg of kola nut extract per day; and group 6 rats were diabetic rats administered 100 mg/kg of kola nut extract per day. Diabetes was induced with 50 mg/kg of STZ. After 3 weeks of administration, the spatial memories of the rats were tested using the Y-maze, followed by assay of Na+/K+-ATPase activity. Results The result shows a significant increase in Na+/K+-ATPase activity of diabetic treated groups (5 and 6) when compared with the diabetic group (4) and a significant increase in Na+/K+-ATPase activity of healthy treated groups (2 and 3) when compared with control. Also, there was a significant increase in spatial memory of the diabetic treated groups when compared with diabetic group. Conclusions This study revealed that kola nut extract has restorative effect on brain Na+/K+-ATPase activities and spatial memory of STZ-induced diabetic female Wistar rats.

Keywords: acetone extract; cognition; diabetes; kola nut; sodium-potassium ATPase.

MeSH terms

  • Acetone / chemistry
  • Animals
  • Brain / drug effects*
  • Brain / metabolism
  • Cola / chemistry*
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / metabolism*
  • Female
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Sodium-Potassium-Exchanging ATPase / metabolism*
  • Spatial Memory / drug effects*
  • Streptozocin / pharmacology

Substances

  • Plant Extracts
  • Acetone
  • Streptozocin
  • Sodium-Potassium-Exchanging ATPase