Innovations in CRISPR technology

Alexandra K Brooks; Thomas Gaj

doi:10.1016/j.copbio.2018.03.007

Innovations in CRISPR technology

Curr Opin Biotechnol. 2018 Aug:52:95-101. doi: 10.1016/j.copbio.2018.03.007. Epub 2018 Apr 5.

Authors

Alexandra K Brooks¹, Thomas Gaj²

Affiliations

¹ Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
² Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Electronic address: gaj@illinois.edu.

PMID: 29626736
DOI: 10.1016/j.copbio.2018.03.007

Abstract

CRISPR-Cas9 is a versatile tool for genome engineering that has revolutionized biotechnology and is poised to impact medicine. Recent advances in the identification of unique CRISPR systems, as well as the re-engineering of the Cas9 protein for expanded function, has enabled the diversification of the CRISPR genome engineering toolbox. In this review, we highlight these innovations and discuss how advances in CRISPR technology can lead to breakthroughs in the field of gene therapy.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biotechnology
CRISPR-Cas Systems / genetics*
Gene Editing
Genetic Techniques*
Genome
Humans
Inventions*