Activated RecA protein may induce expression of a gene that is not controlled by the LexA repressor and whose function is required for mutagenesis and repair of UV-irradiated bacteriophage lambda

J Bacteriol. 1987 Oct;169(10):4816-21. doi: 10.1128/jb.169.10.4816-4821.1987.

Abstract

The activated form of the RecA protein (RecA) is known to be involved in the reactivation and mutagenesis of UV-irradiated bacteriophage lambda and in the expression of the SOS response in Escherichia coli K-12. The expression of the SOS response requires cleavage of the LexA repressor by RecA and the subsequent expression of LexA-controlled genes. The evidence presented here suggests that RecA induces the expression of a gene(s) that is not under LexA control and that is also necessary for maximal repair and mutagenesis of damaged phage. This conclusion is based on the chloramphenicol sensitivity of RecA -dependent repair and mutagenesis of damaged bacteriophage lambda in lexA(Def) hosts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacteriophage lambda / genetics*
  • Bacteriophage lambda / radiation effects
  • Chloramphenicol / pharmacology
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression Regulation*
  • Genes, Bacterial
  • Kinetics
  • Mutation
  • Rec A Recombinases / metabolism*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Serine Endopeptidases*

Substances

  • Bacterial Proteins
  • LexA protein, Bacteria
  • Repressor Proteins
  • Chloramphenicol
  • Rec A Recombinases
  • Serine Endopeptidases