Complete genome sequence of a new bipartite begomovirus infecting Boehmeria leiophylla in China

Arch Virol. 2018 Jul;163(7):1989-1992. doi: 10.1007/s00705-018-3802-1. Epub 2018 Mar 22.

Abstract

A bipartite begomovirus was identified from a Boehmeria leiophylla plant sample exhibiting yellow mosaic symptoms collected in Nabanhe National Nature Reserve, Xishuangbanna, Yunnan, China. Five complete DNA-A and four DNA-B genome sequences were obtained by rolling-circle amplification (RCA), cloned, and sequenced. All DNA-A sequences were determined to be 2759 nucleotides in size, sharing 99.9%-100% nucleotide sequence identity with each other. The DNA-B sequences were comprised of 2673 nucleotides, sharing 98.6-100% nucleotide sequence identity with each other. Genomic organization of the begomovirus was typical of Old World bipartite begomoviruses. Sequence analysis revealed 81.84% nucleotide sequence identity to tomato leaf curl Hsinchu virus (ToLCHsV) from China for the DNA A component and 67.23% identity to the squash leaf curl China virus (SLCCNV) from India for the DNA B component. The sequence comparisons suggest that this bipartite begomovirus represents a novel species for which we propose the name "Ramie yellow mosaic virus".

MeSH terms

  • Base Sequence
  • Begomovirus / genetics*
  • Begomovirus / isolation & purification
  • Boehmeria / virology*
  • China
  • DNA, Viral / genetics
  • Genome, Viral*
  • India
  • Phylogeny
  • Plant Diseases / virology
  • Sequence Analysis, DNA*
  • Whole Genome Sequencing

Substances

  • DNA, Viral

Supplementary concepts

  • Tomato leaf curl virus