Objective: To study the correlation between expression of oncogene C-MYC protein and gene abnormality in diffuse large B-cell lymphoma (DLBCL). Methods: The expression of C-MYC protein and gene abnormality were detected by immunohistochemistry and fluorescence in situ hybridization (FISH), respectively, in 42 cases of paraffin-embedded DLBCL. All cases were collected at Department of Pathology, Weifang People's Hospital during January 2015 to October 2016. Results: The positive rate of C-MYC protein expression was 47.6% (20/42) and the rate of abnormal C-MYC gene by FISH was 26.2%(11/42), including translocation (23.8%, 10/42) and gene amplification (2.4%, 1/42). There was a close relationship between the protein expression and gene translocation (χ(2)=11.813; P=0.001) and gene translocation occurred primarily in GCB (χ(2)=4.029; P=0.045). Conclusion: The high expression (≥40%) of C-MYC protein is associated with its gene translocation, suggesting that C-MYC protein detection can be used as a surrogate marker for C-MYC gene translocation in DLBCL.
目的：探讨弥漫性大B细胞淋巴瘤(DLBCL)中癌基因C－MYC蛋白表达及与基因异常的相关性。 方法：回顾性收集山东省潍坊市人民医院病理科2015年1月至2016年10月首次诊断为DLBCL的石蜡包埋组织标本42例，采用免疫组织化学和荧光原位杂交(FISH)技术检测全部研究对象组织标本中C－MYC蛋白表达与基因异常的情况，观察两者之间的相关性。 结果： C－MYC蛋白表达阳性率为47.6%(20/42)；C－MYC基因异常检出率为26.2%(11/42)，其中基因易位检出率23.8%(10/42)；基因扩增2.4%(1/42)；C－MYC蛋白表达阳性与C－MYC基因易位有相关性(χ(2)＝11.813；P＝0.001)；C－MYC基因易位主要见于生发中心B细胞型(χ(2)＝4.029；P＝0.045)。 结论： C－MYC蛋白表达阳性(≥40%)与C－MYC基因易位有相关性。在DLBCL中，C－MYC蛋白高表达提示可能存在C－MYC基因易位。.
Keywords: Gene amplification; Lymphoma, large B-cell, diffuse; Proto-oncogene proteins c-myc.