DJ-1 is a useful biomarker for invasive extrahepatic cholangiocarcinoma

Hum Pathol. 2018 Jun:76:28-36. doi: 10.1016/j.humpath.2018.02.010. Epub 2018 Feb 13.

Abstract

We have previously reported that DJ-1 protein is up-regulated in cholangiocarcinoma compared with non-neoplastic epithelium of the bile duct in a study using liquid-chromatography mass spectrometry-based proteomics. The aim of this study was to clarify whether DJ-1 expression offers a biomarker for patients with invasive extrahepatic cholangiocarcinoma (EHCC) who undergo surgical resection with curative intent. Positive immunohistochemical (IHC) staining of DJ-1 was significantly more frequent in the cytoplasm of 96 invasive EHCCs (n = 28, 29.2%) than in that of 66 non-neoplastic epithelial lesions adjacent to invasive EHCC (n = 7, 10.6%; P = .006). No significant difference in clinicopathological features was evident between invasive EHCC patients with negative (n = 68) and positive (n = 28) IHC staining. However, negative IHC staining for DJ-1 in cytoplasm was selected as an independent risk factor for adverse prognosis on multivariate analysis (P = .004, hazard ratio 2.13, 95% confidence interval 1.28-3.57). Serum levels of DJ-1 in 16 invasive EHCC patients with metastasis were compared with 12 invasive EHCC patients without metastasis. Serum levels of DJ-1 tended to be higher in 16 patients with metastasis (median, 40.9 ng/ml) than in 12 patients without (27.6 ng/ml, P = .137). In addition, patients with high serum levels (≥ 40 ng/ml) of DJ-1 tended to have metastasis more frequently than those without (P = .054, Fisher's exact test). We concluded that IHC staining pattern and serum level of DJ-1 in patients with invasive EHCC might be predictive of prognosis and metastasis, respectively.

Keywords: Biomarker; DJ-1; ELISA; Extrahepatic cholangiocarcinoma; Immunohistochemical staining; Prognosis.

MeSH terms

  • Aged
  • Bile Duct Neoplasms / blood
  • Bile Duct Neoplasms / enzymology*
  • Bile Duct Neoplasms / pathology
  • Bile Duct Neoplasms / surgery
  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / blood
  • Cholangiocarcinoma / blood
  • Cholangiocarcinoma / enzymology*
  • Cholangiocarcinoma / secondary
  • Cholangiocarcinoma / surgery
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Predictive Value of Tests
  • Prognosis
  • Protein Deglycase DJ-1 / analysis*
  • Protein Deglycase DJ-1 / blood
  • Tissue Array Analysis

Substances

  • Biomarkers, Tumor
  • PARK7 protein, human
  • Protein Deglycase DJ-1