A sensitive electrochemical nonenzymatic biosensor for the detection of H2O2 released from living cells based on ultrathin concave Ag nanosheets

Bo Ma; Chuncai Kong; Xuanxuan Hu; Ke Liu; Qiang Huang; Jian Lv; Wenjing Lu; Xiaojing Zhang; Zhimao Yang; Sen Yang

doi:10.1016/j.bios.2018.01.041

A sensitive electrochemical nonenzymatic biosensor for the detection of H₂O₂ released from living cells based on ultrathin concave Ag nanosheets

Biosens Bioelectron. 2018 May 30:106:29-36. doi: 10.1016/j.bios.2018.01.041. Epub 2018 Jan 31.

Authors

Bo Ma¹, Chuncai Kong², Xuanxuan Hu¹, Ke Liu³, Qiang Huang⁴, Jian Lv¹, Wenjing Lu¹, Xiaojing Zhang¹, Zhimao Yang⁵, Sen Yang⁵

Affiliations

¹ School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
² School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University Suzhou Institute, Suzhou 21500, China. Electronic address: kongcc@xjtu.edu.cn.
³ Hubei Key Laboratory of Advanced Textile Materials & Application, College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430200, China.
⁴ Department of Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shannxi 710004, China.
⁵ School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University Suzhou Institute, Suzhou 21500, China.

PMID: 29414085
DOI: 10.1016/j.bios.2018.01.041

Abstract

Facile synthesis of ultrathin two-dimensional metallic nanosheets with special physical and chemical properties is still challenging. In this work, ultrathin silver nanosheets were synthesized through the galvanic replacement reaction between silver nitrate and Cu microcages without using any surfactant at room temperature. The as-prepared Ag nanosheets (NSs) were concave and had a thickness of sub-10 nm. And the nanosheets exhibited excellent H₂O₂ detection property with a high sensitivity of about 320.3 uA mM^-1 cm^-2 and a low detection limit of 0.17 µM in a wide linear range of 5-6000 μM, and a fast response time (less than 2 s). Besides, the real-time detection of H₂O₂ induced from living HeLa and SH-SY5Y cells were also achieved, indicating the potential application of as-prepared Ag NSs in monitoring physiological and dynamic in-clinic pathological processes.

Keywords: Ag nanosheets; Electrochemical sensor; Hydrogen peroxide; Living cells; Nonenzymatic biosensor.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
HeLa Cells
Humans
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / isolation & purification*
Limit of Detection
Metal Nanoparticles / chemistry
Nanostructures / chemistry*
Silver / chemistry

Substances

Silver
Hydrogen Peroxide