Serotonin receptor 1A promoter polymorphism, rs6295, modulates human anxiety levels via altering parasympathetic nervous activity

Acta Psychiatr Scand. 2018 Mar;137(3):263-272. doi: 10.1111/acps.12853. Epub 2018 Jan 24.

Abstract

Objective: The G-allele of the -1019C/G (rs6295) promoter polymorphism of the serotonin receptor 1A (HTR1A) gene has been implicated in anxiety; however, the underlying neurophysiological processes are still not fully understood. Recent evidence indicates that low parasympathetic (vagal) tone is predictive of anxiety. We thus conducted a structural equation model (SEM) to examine whether the HTR1A rs6295 variant can affect anxiety by altering parasympathetic nervous activity.

Method: A sample of 1141 drug-free healthy Han Chinese was recruited for HTR1A genotyping. Autonomic nervous function was assessed by short-term spectral analysis of heart rate variability (HRV). Anxiety and stress levels were evaluated by the Beck Anxiety Inventory (BAI) and the Perceived Stress Scale (PSS) respectively.

Results: The number of the HTR1A G allele was inversely correlated with high-frequency power (HF), a parasympathetic index of HRV. The HF index was negatively associated with BAI scores. Furthermore, the good-fitting SEM, adjusting for confounding variables (e.g., age and PSS levels), revealed a significant pathway linking rs6295 variant to BAI scores via HF index modulation.

Conclusion: These results are the first to show that HTR1A -1019C/G polymorphism influences anxiety levels by modulating parasympathetic tone, providing a neurophysiological insight into the role of HTR1A in human anxiety.

Keywords: Serotonin 1A receptor (HTR1A) gene; anxiety; heart rate variability (HRV); polymorphism; vagal (parasympathetic) activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anxiety Disorders / genetics*
  • Anxiety Disorders / physiopathology*
  • Cross-Sectional Studies
  • Female
  • Heart Rate / physiology
  • Humans
  • Male
  • Middle Aged
  • Parasympathetic Nervous System / physiopathology*
  • Polymorphism, Single Nucleotide
  • Promoter Regions, Genetic
  • Receptor, Serotonin, 5-HT1A / genetics*
  • Vagus Nerve / physiopathology

Substances

  • HTR1A protein, human
  • Receptor, Serotonin, 5-HT1A