Oral Bifidobacterium longum expressing GLP-2 improves nutrient assimilation and nutritional homeostasis in mice

J Microbiol Methods. 2018 Feb:145:87-92. doi: 10.1016/j.mimet.2018.01.002. Epub 2018 Jan 9.

Abstract

Bifidobacterium has been developed for the oral delivery of peptides and has the added beneficial effect on our bodies through its probiotic properties. Here, we utilize Bifidobacterium as a delivery system to orally deliver Glucagon like peptide-2 (GLP-2). We constructed vector derived from pET-31b(+) to construct a Bifidobacterium longum expressing GLP-2. We then determined the bioactivity of recombinant Bifidobacterium in Caco-2 cells. Finally, we quantified newly synthesized ApoB48 and chylomicron production in mice infused with exogenous GLP-2 or Bifidobacterium expressing GLP-2. Results based on secretion of the triglyceride (TG)-rich lipoprotein (TRL)-ApoB48 and secretion of chylomicron revealed that recombinant Bifidobacterium was efficient in treating intestinal dysfunction,suggesting an alternative way to use Bifidobacterium as a delivery system to deliver GLP-2 for gastrointestinal nutrition coordination.

Keywords: Bifidobacterium longum; Chylomicron; GLP-2; Triglyceride.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Apolipoprotein B-48 / blood
  • Apolipoprotein B-48 / metabolism
  • Bifidobacterium longum / genetics*
  • Bifidobacterium longum / physiology
  • Caco-2 Cells
  • Chylomicrons / blood
  • Chylomicrons / metabolism
  • Glucagon-Like Peptide-2 Receptor / genetics*
  • Glucagon-Like Peptide-2 Receptor / metabolism*
  • Humans
  • Olive Oil / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Triglycerides / blood
  • Triglycerides / metabolism

Substances

  • Apolipoprotein B-48
  • Chylomicrons
  • Glucagon-Like Peptide-2 Receptor
  • Olive Oil
  • Recombinant Fusion Proteins
  • Triglycerides