Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

Marie Holt; Anne Costanzo; Louis Gioia; Brian Abe; Andrew I Su; Luc Teyton

doi:10.1007/978-1-4939-7514-3_14

Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

Methods Mol Biol. 2018:1712:217-238. doi: 10.1007/978-1-4939-7514-3_14.

Authors

Marie Holt¹, Anne Costanzo¹, Louis Gioia², Brian Abe¹, Andrew I Su², Luc Teyton³

Affiliations

¹ Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
² Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.
³ Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. lteyton@scripps.edu.

PMID: 29224077
DOI: 10.1007/978-1-4939-7514-3_14

Abstract

The paucity of pathogenic T cells in circulating blood limits the information delivered by bulk analysis. Toward diagnosis and monitoring of treatments of autoimmune diseases, we have devised single-cell analysis approaches capable of identifying and characterizing rare circulating CD4 T cells.

Keywords: Autoimmunity; CD4 T cells; Microfluidics; Peripheral blood; Single-cell analysis; TCR sequences.

MeSH terms

Animals
Autoimmune Diseases / diagnosis*
Autoimmunity / genetics
CD4-Positive T-Lymphocytes / immunology*
Gene Expression Profiling*
Gene Library
High-Throughput Nucleotide Sequencing*
Humans
Mice
Real-Time Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta / genetics*
Single-Cell Analysis
Statistics as Topic

Substances

Receptors, Antigen, T-Cell, alpha-beta