Establishment of induced pluripotent stem cell line (ZZUi010-A) from an Alzheimer's disease patient carrying an APP gene mutation

Zhilei Wang; Pei Zhang; Yanlin Wang; Changhe Shi; Na Jing; Huifang Sun; Jing Yang; Yutao Liu; Xuejun Wen; Jin Zhang; Shoutao Zhang; Yuming Xu

doi:10.1016/j.scr.2017.10.025

Establishment of induced pluripotent stem cell line (ZZUi010-A) from an Alzheimer's disease patient carrying an APP gene mutation

Stem Cell Res. 2017 Dec:25:213-216. doi: 10.1016/j.scr.2017.10.025. Epub 2017 Nov 3.

Authors

Zhilei Wang¹, Pei Zhang², Yanlin Wang¹, Changhe Shi¹, Na Jing², Huifang Sun¹, Jing Yang¹, Yutao Liu¹, Xuejun Wen³, Jin Zhang⁴, Shoutao Zhang⁵, Yuming Xu⁶

Affiliations

¹ Department of Neurology, The First affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
² School of life sciences, Zhengzhou University, Zhengzhou, Henan 450001, China.
³ Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA, USA; Shanghai East Hospital, The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200120, China.
⁴ Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China.
⁵ School of life sciences, Zhengzhou University, Zhengzhou, Henan 450001, China. Electronic address: zhangst@zzu.edu.cn.
⁶ Department of Neurology, The First affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: xuyuming@zzu.edu.cn.

PMID: 29156377
DOI: 10.1016/j.scr.2017.10.025

Abstract

Alzheimer's disease (AD) is one of the most common neurodegenerative disorders. Previous studies have identified mutations in several genes, such as amyloid precursor protein (APP), presenilin-1 (PSEN1), and presenilin-2 (PSEN2), in patients with early-onset (<65years) familial AD. Recently, a patient with an APP gene mutation was identified; the dermal fibroblasts of the patient were obtained and a line of induced pluripotent stem cells (iPSCs) was successfully generated using the Sendai-virus (SeV) delivery system. The iPSC line will be useful for further study of the pathomechanism and drug screening for AD.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease / genetics*
Alzheimer Disease / metabolism
Amyloid beta-Protein Precursor / genetics*
Amyloid beta-Protein Precursor / metabolism
Cell Line
Female
Fibroblasts / cytology
Fibroblasts / metabolism
Genetic Vectors / genetics
Genetic Vectors / physiology
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Middle Aged
Mutation*
Presenilin-1 / genetics
Presenilin-1 / metabolism
Presenilin-2 / genetics
Presenilin-2 / metabolism
Sendai virus / genetics
Sendai virus / physiology

Substances

Amyloid beta-Protein Precursor
Presenilin-1
Presenilin-2