Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite

Biochem Biophys Res Commun. 2018 Jan 1;495(1):969-975. doi: 10.1016/j.bbrc.2017.11.101. Epub 2017 Nov 16.

Abstract

Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid β secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing.

Keywords: Barnacle; Cement protein; Evolution; Settlement; cp100k.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesives / chemistry*
  • Adhesives / metabolism*
  • Amino Acid Sequence
  • Animals
  • Arthropod Proteins / chemistry*
  • Arthropod Proteins / metabolism*
  • Life Cycle Stages / physiology*
  • Molecular Sequence Data
  • Organ Specificity
  • Thoracica / chemistry*
  • Thoracica / metabolism*
  • Tissue Distribution

Substances

  • Adhesives
  • Arthropod Proteins