High-level of resistance to β-lactam and presence of β-lactamases encoding genes in Ochrobactrum sp. and Achromobacter sp. isolated from soil

J Glob Antimicrob Resist. 2017 Dec:11:133-137. doi: 10.1016/j.jgar.2017.10.014. Epub 2017 Oct 31.

Abstract

Objectives: Bacteria belonging to the genera Ochrobactrum and Achromobacter are bacteria considered opportunistic, causing infections mainly in immunocompromised patients. β-lactamases are the main cause of resistance to β-lactam antibiotics. This study aimed to investigate the antimicrobial resistance profile and the presence of β-lactamases encoding genes in Ochrobactrum sp. and Achromobacter sp. isolated from Brazilian soils.

Methods: Soil samples from the five regions of Brazil were collected for the isolation of bacteria, which were identified molecularly and then, the minimum inhibitory concentration and detection of β-lactamases encoding genes were performed.

Results: High-level of resistance to β-lactam antibiotics and different β-lactamases encoding genes were found (blaCTX-M-Gp1, blaSHV, blaOXA-1-like and blaKPC), including the first report of the presence of blaKPC in bacteria belonging to the genera Ochrobactrum and Achromobacter.

Conclusion: The results showed that the bacteria from this study, belonging to genera Ochrobactrum and Achromobacter isolated from soil, harbor different β-lactamases encoding genes and can act as a reservoir of these genes.

Keywords: Achromobacter sp.; Antimicrobial resistance; Ochrobactrum sp.; Soil; β-Lactamase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achromobacter / genetics
  • Achromobacter / isolation & purification*
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Brazil
  • Microbial Sensitivity Tests
  • Ochrobactrum / genetics
  • Ochrobactrum / isolation & purification*
  • Soil Microbiology
  • beta-Lactam Resistance
  • beta-Lactamases / genetics*
  • beta-Lactams / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactams
  • beta-Lactamases