Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae

Jianli Lin; Nana Huang; Hui Wang; Qinghui Fu; Eryi Wang; Ping Li; Liteng Yang; Xinping Luo; Xiaoyu Liu; Zhigang Liu

doi:10.1016/j.imbio.2017.10.013

Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae

Immunobiology. 2018 Feb;223(2):246-251. doi: 10.1016/j.imbio.2017.10.013. Epub 2017 Oct 5.

Authors

Jianli Lin¹, Nana Huang², Hui Wang², Qinghui Fu², Eryi Wang², Ping Li², Liteng Yang³, Xinping Luo², Xiaoyu Liu⁴, Zhigang Liu⁵

Affiliations

¹ State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China; The Affiliated Luohu Hospital of Shenzhen University, Shenzhen Luohu Hospital Group, Shenzhen 518000, China.
² State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, 3688 Nanhai Ave, Shenzhen 518060, Guangdong, China.
³ The Affiliated Luohu Hospital of Shenzhen University, Shenzhen Luohu Hospital Group, Shenzhen 518000, China.
⁴ State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, 3688 Nanhai Ave, Shenzhen 518060, Guangdong, China. Electronic address: lxy0901@163.com.
⁵ State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China; State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, 3688 Nanhai Ave, Shenzhen 518060, Guangdong, China. Electronic address: lzg195910@126.com.

PMID: 29102047
DOI: 10.1016/j.imbio.2017.10.013

Abstract

House dust mite (HDM) allergen is a major cause of allergic disease. In this study, two-dimensional immunoblot and Matrix-Assisted Laser Desorption Ionization tandem Time-of-flight mass spectrometry (MALDI-TOF-MS) were used to identify Der f 31. After Der f 31 was cloned, expressed and purified, skin prick test (SPT), Immune inhibitory assays, Western blot, ELISA and asthmatic mouse model were employed to examine the allergenicity of recombinant Der f 31. The gene of Der f 31 includes 447 bps, and encoded 148 amino acids. Positive responses of SPT to r-Der f 31 were 32.5% in 43 HDM-allergic patients. r-Der f 31 can induce allergic pulmonary inflammation in the mouse model. In conclusion, Der f 31 is a novel subtype of dust mite allergens.

Keywords: Allergenomics; Cofilin; Der f 31; Dermatophagoides farinae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Depolymerizing Factors / chemistry
Actin Depolymerizing Factors / immunology
Adolescent
Adult
Aged
Allergens / immunology*
Animals
Antigens, Dermatophagoides / immunology*
Asthma / immunology*
Child
Dermatophagoides farinae / immunology*
Disease Models, Animal
Female
Humans
Hypersensitivity / immunology*
Immunoglobulin E / blood
Male
Mice
Middle Aged
Skin Tests
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Young Adult

Substances

Actin Depolymerizing Factors
Allergens
Antigens, Dermatophagoides
Immunoglobulin E