Substrate-mediated gene transduction of LAMA3 for promoting biological sealing between titanium surface and gingival epithelium

Abstract

A good biological sealing between soft tissues and an implant could minimize the risk of peri-implantitis. Like the junctional epithelium, the peri-implant epithelium attaches to the surface of the implant via hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL). A multilayer coating modified with the laminin332 gene (LAMA3) on the titanium implant surface is developed here via layer-by-layer assembly and antibody-antigen specific binding for substrate-mediated gene transduction. The results in vitro indicated that the LAMA3-modified coating on the titanium surface could improve HaCaT cell adhension in the early stage, and promote the expression of laminin α3 on both the protein and the gene levels. Moreover, the formation of hemidesmosomes at the interface became obvious. In vivo experiments demonstrated that the LAMA3 gene coating on the implant surface could enhance the expression of laminin α3 and improve the biological sealing between the implant and the epithelium. The success of the LAMA3 functionalized multilayer coating in improving biological sealing between titanium implant and gingival epithelium might provide a new approach and experimental evidence for research on the interface of the implant and soft tissues.

Keywords: Biological sealing; Gingival epithelium; Hemidesmosomes; Laminin332; Substrate-mediated gene transduction; Titianium implant.