Abstract
Circulating microRNAs are increasingly being used as noninvasive prognostic and predictive biomarkers for various types of cancer. We describe a method that uses real-time quantitative PCR (qPCR) for establishing a signature plasma microRNA profile that can distinguish patients with osteosarcoma from healthy control samples.
Keywords:
Biomarker; MicroRNA; Osteosarcoma; Plasma; Real-time quantitative PCR.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / blood*
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Biomarkers, Tumor / genetics
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Bone Neoplasms / blood
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Bone Neoplasms / diagnosis*
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Female
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Gene Expression Profiling / methods*
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Humans
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MicroRNAs / blood*
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MicroRNAs / genetics
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Osteosarcoma / blood
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Osteosarcoma / diagnosis*
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Prognosis
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Real-Time Polymerase Chain Reaction
Substances
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Biomarkers, Tumor
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MicroRNAs