Restriction fragment length polymorphisms of horse class II MHC genes observed using various human alpha- and beta-chain cDNA probes

K Hänni; F Hesford; S Lazary; H Gerber

doi:10.1111/j.1365-2052.1988.tb00831.x

Restriction fragment length polymorphisms of horse class II MHC genes observed using various human alpha- and beta-chain cDNA probes

Anim Genet. 1988;19(4):395-408. doi: 10.1111/j.1365-2052.1988.tb00831.x.

Authors

K Hänni¹, F Hesford, S Lazary, H Gerber

Affiliation

¹ Klinik fur Nutztiere und Pferde, Institute of Animal Husbandry, University of Berne, Switzerland.

PMID: 2906789
DOI: 10.1111/j.1365-2052.1988.tb00831.x

Abstract

Genomic DNA isolated from 20 horses was digested with up to six restriction endonucleases and subjected to southern blot hybridization analysis using various human class II alpha- and beta-chain cDNA probes. A high degree of restriction fragment length polymorphism (RFLP) was found for the DQ alpha, DP beta, DQ beta and DR beta probes, about 20 polymorphic bands being detected for each. DR alpha showed 2-4 polymorphic bands, whereas no evidence for DP alpha-like genes was found. A number of correlations of RFLPs with individual alloantisera were apparent.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Southern / veterinary
DNA
DNA Probes
Female
Histocompatibility Antigens Class II / genetics*
Histocompatibility Antigens Class II / immunology
Horses / genetics*
Horses / immunology
Humans
Male
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*

Substances

DNA Probes
Histocompatibility Antigens Class II
DNA