Vitamin E-Coated Dialyzer Inhibits Oxidative Stress

Abstract

Aim: To examine the effects of vitamin E-coated dialyzer on oxidative stress in vitro.

Methods: A dialyzer with a synthetic polymer membrane (APS-11SA) and vitamin E-coated dialyzer (VPS-11SA) were connected to a blood tubing line, and U937 cells were circulated in the device. The circulating fluid was collected at 1, 2, 5, 10, 25, and 50 cycles, which are estimated numbers of passes through the dialyzer. Intracellular reactive oxygen species (ROS) production, malondialdehyde (MDA), and Cu/Zn-superoxide dismutase (SOD) were quantified.

Results: Intracellular ROS production was increased in the first cycle by APS-11SA and was decreased throughout the experiment by VPS-11SA. Intracellular ROS production in the VPS-11SA device was lower, and MDA levels were decreased. MDA levels were lower during VPS-11SA processing than during APS-11SA processing. Cu/Zn-SOD levels remained unchanged.

Conclusion: Our results highlight anti-oxidative-stress effects of a vitamin E-coated dialyzer.

Keywords: Anti-oxidative stress effect; Cu/Zn-superoxide dismutase; Intracellular reactive oxygen species production; Malondialdehyde; Synthetic polymer membrane; Vitamin E-coated dialyzer.