Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses

Feng Cong; Yujun Zhu; Xiangnan Liu; Xiuzhen Li; Meili Chen; Ren Huang; Pengju Guo

doi:10.1016/j.mcp.2017.10.002

Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses

Mol Cell Probes. 2018 Feb:37:1-5. doi: 10.1016/j.mcp.2017.10.002. Epub 2017 Oct 18.

Authors

Feng Cong¹, Yujun Zhu¹, Xiangnan Liu², Xiuzhen Li¹, Meili Chen³, Ren Huang³, Pengju Guo⁴

Affiliations

¹ Guangdong Laboratory Animals Monitoring Institute, Guangzhou 5106, China.
² Guangdong Laboratory Animals Monitoring Institute, Guangzhou 5106, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
³ Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 5106, China.
⁴ Guangdong Laboratory Animals Monitoring Institute, Guangzhou 5106, China. Electronic address: guo_pengju@hotmail.com.

PMID: 29054443
DOI: 10.1016/j.mcp.2017.10.002

Abstract

Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 × 10²- 3.52 × 10³copies/μL. We examined 60 clinical specimens and found 18 positive for respiratory viruses. Our result demonstrated that xTAG-multiplex PCR method is a high-throughput, rapid, specific and sensitive assay for use in epidemiological studies and clinical detection of avian respiratory pathogens.

Keywords: AIV; IBV; ILTV; Luminex; NDV; xTAG-multiplex PCR.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Virus Infections / diagnosis
DNA Virus Infections / veterinary
DNA Virus Infections / virology
Herpesvirus 1, Gallid / genetics
Herpesvirus 1, Gallid / isolation & purification*
Infectious bronchitis virus / genetics
Infectious bronchitis virus / isolation & purification*
Influenza A virus / genetics
Influenza A virus / isolation & purification*
Limit of Detection
Multiplex Polymerase Chain Reaction / methods*
Newcastle disease virus / genetics
Newcastle disease virus / isolation & purification*
Poultry
Poultry Diseases / diagnosis*
Poultry Diseases / virology
RNA Virus Infections / diagnosis
RNA Virus Infections / veterinary
RNA Virus Infections / virology
Sensitivity and Specificity