Guidelines for the use of flow cytometry and cell sorting in immunological studies

Affiliations

• ¹ Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy.
• ² Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
• ³ Swiss Institute of Allergy and Asthma Research (SIAF), University Zurich, Davos, Switzerland.
• ⁴ Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
• ⁵ University of Florence, Internal Medicine, Florence, Italy.
• ⁶ Charité - Universitätsmedizin Berlin, Germany.
• ⁷ Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Via Regina Elena 324, 00161 Rome, Italy.
• ⁸ Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Rome, Italy.
• ⁹ Neuroimmunology and Flow Cytometry Units, Santa Lucia Foundation, Rome, Italy.
• ¹⁰ Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
• ¹¹ University of Zurich, Institute of Experimental Immunology, Zürich, Switzerland.
• ¹² Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health.
• ¹³ Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland.
• ¹⁴ Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
• ¹⁵ The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, New York, USA.
• ¹⁶ Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC, Canada.
• ¹⁷ Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.
• ¹⁸ Biopyhsics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
• ¹⁹ DZIF - National Centre for Infection Research, Munich, Germany.
• ²⁰ Focus Group ''Clinical Cell Processing and Purification", Institute for Advanced Study, Technische Universität München, Munich, Germany.
• ²¹ Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester NY, United States of America.
• ²² Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
• ²³ National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
• ²⁴ Department of Immunology & Center for Immunotherapy, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China.
• ²⁵ INMP/NIHMP, Rome, Italy.
• ²⁶ Immunotechnology Section, Vaccine Research Center, NIH, Bethesda, MD, USA.
• ²⁷ Medizinische Klinik mit Schwerpunkt Rheumatologie und Medizinische Immunolologie Charité Universitätsmedizin Berlin, Berlin, Germany.
• ²⁸ Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada.
• ²⁹ University of Milano and Don C Gnocchi Foundation IRCCS, Milano, Italy.
• ³⁰ Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
• ³¹ CEA - Université Paris Sud - INSERM U, Immunology of viral infections and autoimmune diseases, France.
• ³² Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italia.
• ³³ Lymphopoiesis Unit, Immunology Department Pasteur Institute, Paris, France.
• ³⁴ Flow Cytometry Facility, The Francis Crick Institute, London, United Kingdom.
• ³⁵ Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
• ³⁶ Istituto Giannina Gaslini, Genova, Italy.
• ³⁷ University of Milan, Department of Medical Biotechnologies and Translational Medicine.
• ³⁸ Humanitas Clinical and Research Center, Lab of Clinical and Experimental Immunology, Rozzano, Milan, Italy.
• ³⁹ Experimental Immunology Unit, Head, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy.
• ⁴⁰ Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey.
• ⁴¹ Sony Europe Ltd, Weybridge, UK.
• ⁴² Institut Pasteur, Immunology, Paris, France.
• ⁴³ University of Palermo, Department of Biopathology, Palermo, Italy.
• ⁴⁴ Institute of Experimental Immunology, University Bonn, Bonn, Germany.
• ⁴⁵ Department of Cardiothoracic Surgery, School of Medicine, University of Pittsburgh, PA.
• ⁴⁶ Department of Medicine/Rheumatology and Clinical Immunology, Charite Universitätsmedizin Berlin, Germany.
• ⁴⁷ Department of Immunology, University of Toronto, Toronto, Canada.
• ⁴⁸ Department of Molecular Medicine and Experimental Immunology, (Core Facility Flow Cytometry) University of Bonn, Germany.
• ⁴⁹ Department of Biomedical Sciences, University of Barcelona, Barcelona, Spain.
• ⁵⁰ Professor for Immunobiology, Director, Theodor Kocher Institute, University of Bern, Bern, Switzerland.
• ⁵¹ IUF - Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany.
• ⁵² Leiden University Medical Center, Department of Parasitology, Leiden, The Netherlands.
• ⁵³ Institute of Transplant Immunology, IFB-Tx, MHH Hannover Medical School, Hannover, Germany.
• ⁵⁴ German Center for Infectious diseases (DZIF), TTU-IICH, Hannover, Germany.
• ⁵⁵ Divisions of Hematology & Oncology, Department of Medicine, Washington University School of Medicine, St Louis, MO.
• ⁵⁶ The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
• ⁵⁷ Institut Necker-Enfants Malades (INEM), INSERM U-CNRS UMR, Paris, France.
• ⁵⁸ Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.
• ⁵⁹ Assistance Publique - Hôpitaux de Paris (AP-HP), Hôpital Necker Enfants Malades, Paris, France.
• ⁶⁰ Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
• ⁶¹ Immunology and Environment, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.
• ⁶² Owl Biomedical Inc., Santa Barbara, USA.
• ⁶³ John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.
• ⁶⁴ Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.
• ⁶⁵ University of Arizona, Bio Institute, School of Plant Sciences and Arizona Cancer Center, Tucson, Arizona, USA.
• ⁶⁶ Department of Molecular Immunology, Institute of Experimental Immunology, Bonn, Germany.
• ⁶⁷ Josep Carreras Leukemia Research Institute, Barcelona, Spain.
• ⁶⁸ INGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
• ⁶⁹ Flow Cytometry Core Facility, Department of Dermatology, University Medical Center, Eberhard Karls University Tübingen, Germany.
• ⁷⁰ Institut fuer Genetik, Universitaet zu Koeln, Koeln, Germany.
• ⁷¹ Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow.
• ⁷² Clinic of Infectious Diseases, "San Gerardo" Hospital - ASST Monza, University Milano-Bicocca, Monza, Italy.
• ⁷³ Genentech, Department of Cancer Immunology, South San Francisco, California, USA.
• ⁷⁴ Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
• ⁷⁵ Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Internal Medicine, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
• ⁷⁶ Immundynamics, Charité - Universitätsmedizin Berlin, Berlin, Germany.
• ⁷⁷ Houston Methodist Hospital Research Institute, Houston, TX, USA.
• ⁷⁸ Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, The University of Valencia. Av. Blasco Ibáñez, Valencia, Spain.
• ⁷⁹ Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands.
• ⁸⁰ Chemical and Materials Engineering, New Mexico State University, Las Cruces, NM, 88003, USA.
• ⁸¹ Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
• ⁸² Department of Immunology, Institute of Basic Medical Sciences & State Key Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
• ⁸³ Clinical Immunology Center, Chinese Academy of Medical Sciences, Beijing, China.
• ⁸⁴ Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
• ⁸⁵ Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy.
• ⁸⁶ Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of MolecularMedicine, University Hospital Erlangen, Erlangen, Germany.
• ⁸⁷ Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, The University of Valencia. Av. Blasco Ibáñez, Valencia, Spain.
• ⁸⁸ Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia.
• ⁸⁹ Department for Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
• ⁹⁰ Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.
• ⁹¹ Center for Chronic Immunodeficiency (CCI), Faculty of Medicine, University of Freiburg, Freiburg, Germany.
• ⁹² Medical University of Graz, Institute of Cell Biology, Histology & Embryology, Graz, Austria.
• ⁹³ BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin.
• ⁹⁴ Division of immunology, the Netherlands Cancer Institute, Amsterdam.
• ⁹⁵ University of Virginia School of Medicine, Flow Cytometry Shared Resource, Charlottesville, VA, USA.
• ⁹⁶ INSERM U932, Institut Curie, Paris 75005, France.
• ⁹⁷ Laboratoire d'immunologie clinique, Institut Curie, Paris 75005, France.
• ⁹⁸ Centre d'investigation Clinique en Biothérapie Gustave-Roussy Institut Curie (CIC-BT1428), Institut Curie, Paris 75005, France.
• ⁹⁹ Singapore Immunology Network (SIgN), Principal Investigator, Biology of Aging Program.
• ¹⁰⁰ Director Flow Cytomerty Platform, Immunomonitoring Platform, Agency for Science Technology and Research (A*STAR), Singapore.
• ¹⁰¹ Department of Medicine, University of Sherbrooke, Qc, Canada.
• ¹⁰² Faculty of Sciences, ElManar University, Tunis, Tunisia.
• ¹⁰³ Section of Immunology, Vetsuisse Faculty, University of Zurich, Switzerland.
• ¹⁰⁴ The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, CA, USA.
• ¹⁰⁵ Department of Surgery, University of British Columbia & British Columbia Children's Hospital Research Institute, Vancouver, BC, Canada.
• ¹⁰⁶ Covance, Hematology, Indianapolis, IN, USA.
• ¹⁰⁷ Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg 35043, Germany.
• ¹⁰⁸ MRC Centre for Transplantation, King's College London, Guy's Hospital, SE1 9RT London, UK.
• ¹⁰⁹ Beckman Coulter, Inc, Miami, FL, USA.
• ¹¹⁰ Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USA.
• ¹¹¹ Erasmus MC, University Medical Center, Department of Rheumatology, Rotterdam, The Netherlands.
• ¹¹² Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
• ¹¹³ Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
• ¹¹⁴ Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
• ¹¹⁵ Department of Virus Immunology, Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
• ¹¹⁶ Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy.
• ¹¹⁷ Senior Group on Lymphocyte Development, Max Planck Institute for Infection Biology, Berlin, Germany.
• ¹¹⁸ Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany.
• ¹¹⁹ Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
• ¹²⁰ Trinity Biomedical Sciences Institute, Trinity College Dublin, the University of Dublin, Dublin, Ireland.
• ¹²¹ Center for Infectious Medicine, Department of Medicine, Karolinska Institute Stockholm, Sweden.
• ¹²² Department of Clinical and Experimental Medicine, Linköping University, Sweden.
• ¹²³ Department of Pathology and Laboratory Medicine, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, Pennsylvania.
• ¹²⁴ Department of Experimental Medicine, University of Genova, Genova, Italy.
• ¹²⁵ Centro di Eccellenza per la Ricerca Biomedica-CEBR, Genova, Italy.
• ¹²⁶ Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy.
• ¹²⁷ David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
• ¹²⁸ Centre for Environmental Research - UFZ, Department Environemntal Microbiology, Leipzig, Germany.
• ¹²⁹ Bill Ford Chair in Cellular Immunology, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.
• ¹³⁰ Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München (TUM), Munich, Germany.
• ¹³¹ Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany.
• ¹³² Department of Pathology and Immunology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
• ¹³³ Howard Hughes Medical Institute, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
• ¹³⁴ Department of Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.
• ¹³⁵ The Scintillon Institute, Nancy Ridge Drive, San Diego, CA, USA.
• ¹³⁶ Centre for Microvascular Research, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.
• ¹³⁷ Department of Inflammation and Oncology, Amgen Inc., South San Francisco, CA, USA.
• ¹³⁸ Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
• ¹³⁹ Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17489, Greifswald, Germany.
• ¹⁴⁰ Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
• ¹⁴¹ Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
• ¹⁴² Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
• ¹⁴³ Department of Life Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
• ¹⁴⁴ Chromocyte Limited, Electric Works, Sheffield, UK.
• ¹⁴⁵ Univeristy of Udine - Department of Medicine, Lab of Immunology, Udine, Italy.
• ¹⁴⁶ Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands; Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
• ¹⁴⁷ Bindley Biosciences Center, Purdue University, West Lafayette, In, USA.
• ¹⁴⁸ Desatoya LLC, Reno, NV, USA.
• ¹⁴⁹ Department of Experimental Immunology and Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, The Netherlands.
• ¹⁵⁰ Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
• ¹⁵¹ The SVM Professor of Cytomics & Professor of Biomedical Engineering, Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, USA.
• ¹⁵² Cell Biology Unit, IRCCS AOU San Martino-IST, Genova, Italy.
• ¹⁵³ Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
• ¹⁵⁴ German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
• ¹⁵⁵ German Center for Infection Research (DZIF), partner site Munich, Munich, Germany.
• ¹⁵⁶ Laboratory of Experimental Immunology, WPI Immunology Frontier Research Center (IFReC), Osaka University, Suita 565-0871, Japan.
• ¹⁵⁷ Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
• ¹⁵⁸ Institute of Immunology, Section Molecular Immunology, Ruprecht-Karls-University, D-69120, Heidelberg, Germany.
• ¹⁵⁹ Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology,Oxford, United Kingdom.
• ¹⁶⁰ Charité-Universitaetsmedizin Berlin, Corporate Member of Freie Universitaet Berlin, Humboldt-Universitaet zu Berlin.
• ¹⁶¹ Berlin Institute of Health, Institute of Medical Immunology, Augustenburger Platz 1, 13353 Berlin, Germany.
• ¹⁶² Harvard Medical School, Department of Microbiology and Immunobiology, Boston, MA, USA.
• ¹⁶³ R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
• ¹⁶⁴ Klinik und Poliklinik für Innere Medizin I, Universitätsklinikum Regensburg, Regensburg, Germany.
• ¹⁶⁵ Imaging and Cytometry Core Facility, Flow Cytometry Unit, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
• ¹⁶⁶ Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany.
• ¹⁶⁷ AsedaSciences, West Lafayette, IN, USA.
• ¹⁶⁸ BD Life Sciences, San Diego, CA USA.
• ¹⁶⁹ Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
• ¹⁷⁰ Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima, Japan.
• ¹⁷¹ Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
• ¹⁷² Institute for Medical Informatics, IMISE, Leipzig, Germany.
• ¹⁷³ School of Life Sciences and Medical Center, Institute of Immunology, Key Laboratory of Innate Immunity and Chronic Disease of Chinese Academy of Science, University of Science and Technology of China, Hefei, China.
• ¹⁷⁴ Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
• ¹⁷⁵ University of Birmingham, Institute of Immunology and Immunotherapy, Birmingham, UK.
• ¹⁷⁶ Novartis Institute for Biomedical Research (NIBR), Basel Switzerland.
• ¹⁷⁷ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo.
• ¹⁷⁸ Instituto de Medicina Molecular, Lisbon, Portugal.
• ¹⁷⁹ Unité Lymphopoiese, Institut Pasteur, Paris, France.
• ¹⁸⁰ Department of Infection Biology, University Hospital Erlangen, Wasserturmstr. 3/5, 91054 Erlangen, Germany.
• ¹⁸¹ Inst. Med. Immunol. & BCRT & Labor Berlin.
• ¹⁸² A·P·E-Applied Physics & Electronics, Berlin, Germany.
• ¹⁸³ Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
• ¹⁸⁴ Babraham Institute, Cambridge, UK.
• ¹⁸⁵ Thermo Fisher Scientific, Eugene, Oregon, USA.
• ¹⁸⁶ Medinfomatics Ltd., West Drayton, Middlesex, England.
• ¹⁸⁷ Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, IfADo, Department of Immunology, Dortmund, Germany.
• ¹⁸⁸ Universitätsmedizin Göttingen, Georg-August-Universität, Abt. Zelluläre und Molekulare Immunologie, Humboldtallee 34, 37073 Göttingen, Germany.
• ¹⁸⁹ Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany.
• ¹⁹⁰ BD Biosciences, San Jose CA, USA.
• ¹⁹¹ School of Computing Science, Simon Fraser University, Burnaby, Canada.
• ¹⁹² Emerald Biotech Co., Ltd, Hangzhou, China.
• ¹⁹³ Department of Rheumatology & Immunology, West China Hospital, Sichuan University, Chengdu, China.
• ¹⁹⁴ Maurice Müller Laboratories (DKF), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Murtenstrasse, Bern.