Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)

Methods Mol Biol. 2018:1692:129-138. doi: 10.1007/978-1-4939-7401-6_12.

Abstract

Epithelial Mesenchymal Transition (EMT) is a key event in cancer progression. During this event, epithelial cancer cells undergo molecular and cellular changes leading to their trans-differentiation into mesenchymal cancer cells that are capable of migration, invasion, and metastasis to other tissues and organs. Here, we present a method for in vitro induction of EMT in prostate cancer cell lines using lentiviral expression of a PMLI isoform mutant construct.

Keywords: E-Cadherin; Epithelial cells; Epithelial mesenchymal transition (EMT); Lentiviral expression system; Mesenchymal cells; Mutagenesis; N-Cadherin; Promylocytic leukemia protein (PML); Prostate cancer (PCa); Vimentin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadherins / metabolism
  • Cell Line, Tumor
  • Cytoplasm / metabolism*
  • Epithelial-Mesenchymal Transition / genetics
  • Epithelial-Mesenchymal Transition / physiology*
  • Gene Expression Regulation, Neoplastic / genetics
  • Gene Expression Regulation, Neoplastic / physiology
  • Humans
  • Male
  • Mutation / genetics
  • Promyelocytic Leukemia Protein / genetics*
  • Promyelocytic Leukemia Protein / metabolism*
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / metabolism*
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Vimentin / metabolism

Substances

  • Cadherins
  • Promyelocytic Leukemia Protein
  • Protein Isoforms
  • Vimentin
  • PML protein, human