Immunohistochemical Comparison of Malignancy Between Radial Invasion and Mucosal Extension in Hilar Cholangiocarcinoma

Anticancer Res. 2017 Oct;37(10):5805-5812. doi: 10.21873/anticanres.12023.

Abstract

Aim: To compare the cells of mucosal extension (ME) and radial invasion (RI) in hilar cholangiocarcinoma (HCCA) for optimal resection.

Materials and methods: Forty-six patients underwent surgery for HCCA between 1992 and 2004. Immunohistochemical expressions of p53, Ki-67, matrix metalloproteinase-7 (MMP7), mucin 1 (MUC1), and E-cadherin were assessed at five different sites of the tumour and compared between the recurrence and non-recurrence groups.

Results: Expression of E-cadherin was significantly lower in RI cells than in ME cells, and that of MMP7 and MUC1 was significantly higher in RI cells than in ME cells. Ki-67 expression was higher in ME cells than in RI cells. During the 11-year follow-up, recurrence in patients with R0 resection was associated with significantly lower E-cadherin, higher MMP7, and higher Ki-67 expression.

Conclusion: Removal of as many RI cells as possible should be a priority in resection of HCCA, followed by removal of ME cells. E-Cadherin appears to be associated with recurrence of HCCA.

Keywords: E-cadherin; Hilar cholangiocarcinoma; Ki-67; MMP7; MUC1; recurrence.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Antigens, CD
  • Bile Duct Neoplasms / chemistry*
  • Bile Duct Neoplasms / pathology*
  • Bile Duct Neoplasms / surgery
  • Biomarkers, Tumor / analysis*
  • Cadherins / analysis
  • Female
  • Humans
  • Immunohistochemistry*
  • Ki-67 Antigen / analysis
  • Klatskin Tumor / chemistry*
  • Klatskin Tumor / pathology*
  • Klatskin Tumor / surgery
  • Male
  • Matrix Metalloproteinase 7 / analysis
  • Middle Aged
  • Mucin-1 / analysis
  • Mucous Membrane / pathology*
  • Neoplasm Invasiveness
  • Neoplasm Recurrence, Local
  • Predictive Value of Tests
  • Retrospective Studies
  • Risk Factors
  • Time Factors
  • Treatment Outcome
  • Tumor Suppressor Protein p53 / analysis

Substances

  • Antigens, CD
  • Biomarkers, Tumor
  • CDH1 protein, human
  • Cadherins
  • Ki-67 Antigen
  • MUC1 protein, human
  • Mucin-1
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • MMP7 protein, human
  • Matrix Metalloproteinase 7