hsa-miR-1973 MicroRNA is Significantly and Differentially Expressed in MDA-MB-231 Cells of Breast Adenocarcinoma and Xenografts Derived from the Tumor

Bull Exp Biol Med. 2017 Sep;163(5):660-662. doi: 10.1007/s10517-017-3873-0. Epub 2017 Sep 26.

Abstract

The gene expression profiles of MDA-MB-231 cell line of breast cancer and xenografts derived from this tumor in murine model were analyzed using GeneChip Human Transcriptome array 2.0 platform (Affymetrix). A more than 1000-fold increase in the MIR1973 gene expression was observed in the xenografts compared to the original cell line. Real-time reverse transcription PCR showed that the content of mature hsa-miR-1973 microRNA encoded by this gene was elevated in the xenografts by more than 300 times. According to microarray analysis, none of hsa-miR-1973 target genes available in the databases changed the expression in the cell line and xenografts. A possible role of hsa-miR-1973 is reduction of apoptosis in the tumor.

Keywords: MDA-MB-231; breast cancer; cell line; microRNA; xenograft.

MeSH terms

  • Adenocarcinoma / genetics*
  • Animals
  • Breast Neoplasms / genetics*
  • Cell Line, Tumor
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / genetics*
  • Gene Expression Regulation, Neoplastic / physiology
  • Humans
  • Mice
  • Mice, SCID
  • MicroRNAs / genetics*
  • MicroRNAs / physiology
  • Xenograft Model Antitumor Assays

Substances

  • MicroRNAs