Detection and genomic characterization of a mammary-like adenocarcinoma

Cold Spring Harb Mol Case Stud. 2017 Nov 21;3(6):a002170. doi: 10.1101/mcs.a002170. Print 2017 Nov.

Abstract

Whole-genome and transcriptome sequencing were performed to identify potential therapeutic strategies in the absence of viable treatment options for a patient initially diagnosed with vulvar adenocarcinoma. Genomic events were prioritized by comparison against variant distributions in the TCGA pan-cancer data set and complemented with detailed transcriptome sequencing and copy-number analysis. These findings were considered against published scientific literature in order to evaluate the functional effects of potentially relevant genomic events. Analysis of the transcriptome against a background of 27 TCGA cancer types led to reclassification of the tumor as a primary HER2+ mammary-like adenocarcinoma of the vulva. This revised diagnosis was subsequently confirmed by follow-up immunohistochemistry for a mammary-like adenocarcinoma. The patient was treated with chemotherapy and targeted therapies for HER2+ breast cancer. The detailed pathology and genomic findings of this case are presented herein.

Keywords: neoplasm of the breast; neoplasm of the genitourinary tract.

Publication types

  • Case Reports

MeSH terms

  • Adenocarcinoma / genetics*
  • Breast / pathology
  • Breast Neoplasms / genetics
  • Diagnosis, Differential
  • Female
  • Gene Expression Profiling
  • Genomics
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Receptor, ErbB-2 / genetics
  • Receptor, ErbB-2 / metabolism
  • Transcriptome
  • Vulva / pathology*
  • Vulvar Neoplasms / genetics*
  • Whole Genome Sequencing

Substances

  • ERBB2 protein, human
  • Receptor, ErbB-2