Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae

J Microbiol Methods. 2017 Nov:142:1-3. doi: 10.1016/j.mimet.2017.08.016. Epub 2017 Aug 24.

Abstract

The rapid detection of KPC-producing Enterobacteriaceae by microbiology laboratories has been required for infectious control programs. Herein we evaluated the performance of a novel immunochromatographic test for detecting KPC-2-, KPC-3-, KPC-4-, KPC-6-, KPC-7-, KPC-8-, and KPC-11-producing isolates and the influence of different growth media on the test performance.

Keywords: Chromogenic agar; Culture medium; KPC variants; KPC-K-SeT; MacConkey; Mueller-Hinton.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / genetics*
  • Carbapenem-Resistant Enterobacteriaceae / enzymology
  • Carbapenem-Resistant Enterobacteriaceae / genetics*
  • Carbapenem-Resistant Enterobacteriaceae / isolation & purification
  • Chromatography, Affinity / methods*
  • Enterobacteriaceae Infections / microbiology
  • Humans
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Microbial Sensitivity Tests
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase